XL2-Blue MRF’ 感受态细胞

出品公司: Stratagene
菌株类型: E.coli
培养基: LB培养基
生长条件: 37 ℃,  有氧
基因型: D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F¢proAB lacIqZDM15 Tn10 (Tetr) Amy Camr]
抗性: 氯霉素,四环素
质粒转化条件: 42 ℃热激
应用: 蛋白表达
产品编号 产品名称 规格 价格
ST1057 XL2-Blue MRF’



XL2-Blue MRF´ ultracompetent cells are high-efficiency derivatives of our XL1-Blue MRF´ supercompetent cells.  Using ultracompetent cell technology, we have achieved transformation efficiencies of greater than 5 × 109 transformants/µg of pUC18 DNA, making these ultracompetent cells an ideal choice for high-efficiency cloning. The XL2-Blue MRF´ (Minus Restriction) strain is deficient in all known restriction systems (McrA–, McrCB–, McrF–, Mrr–, HsdR–), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA.   XL2-Blue MRF´ cells are restriction minus. The strain is endonuclease deficient (endA), greatly improving the quality of miniprep DNA, and recombination deficient (recA), helping to ensure insert stability. These cells allow blue-white screening for recombinant plasmids.