p3xFLAG-CMV-10 载体

质粒类型: 哺乳动物表达载体
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6299 bp (查看载体序列)
5' 测序引物及序列: T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
载体标签: 3xFLAG
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neo,G418
产品编号 产品名称 规格 价格
VT1070 p3xFLAG-CMV-10 2ug 点击询价

The p3XFLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.