pET-41Ek/LIC 载体

质粒类型: 大肠杆菌表达载体
表达水平: 高拷贝
载体大小: 5947 bp (查看载体序列)
5' 测序引物 T7 Fwd
5' 测序引物序列: 5'd[TAATACGACTCACTATAGGG]3'
载体标签: GST (Nterm), His (Nterm), S-Tag (Cterm)
载体抗性: Kanamycin (卡那霉素)
备注: For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site
产品编号 产品名称 规格 价格
VT1226 pET-41Ek/LIC 2ug 点击询价

The pET-41 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal GST•Tag™, His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-41 Ek/LIC Vector Kit (Cat. No. 71071-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3). Vector encoded sequence can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.