pCDFDuet-1 载体

基本信息

别名: pCDFDuet1, pCDFDuet 1
质粒类型: 大肠杆菌表达载体（双启动子）
表达水平: 高
克隆方法: 多克隆位点，限制性内切酶
载体大小: 3781 bp (查看载体序列)
5' 测序引物: T7
5' 测序引物序列:   T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物: T7t
3' 测序引物序列:   T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签: N-His, C-S
载体抗性: streptomycin
备注:
pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).

订购信息

产品编号 产品名称 规格 价格
VT1239 pCDFDuet-1 2ug 点击询价

质粒图谱

pCDFDuet-1 质粒图谱
pCDFDuet-1 Vector Map

载体描述

载体说明

pCDFDuet™-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.