pRNATin-H1.2/Retro 载体

基本信息

质粒类型: RNAi载体
启动子: H1.2
克隆方法: 多克隆位点，限制性内切酶
载体大小: 7706 bp (查看载体序列)
5' 测序引物及序列: CCAACTTGTTTATTGCAGCT 
3' 测序引物及序列: TAATACGACTCACTATAGGG 
载体标签: cGFP
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin (新霉素)

订购信息

产品编号 产品名称 规格 价格
VT1435 pRNATin-H1.2/Retro 2ug 点击询价

质粒图谱

pRNATin-H1.2/Retro 质粒图谱

载体描述

pRNATin-H1.2/Retro siRNA expression vector is compatible with Clontech Retro-X Expression System. It uses an inducible H1 promoter for siRNA expression. The promoter contains the tetracycline operator (TetO1). As a competitor, tetracycline or doxycycline can bind this operator to remove the blockade of tetracycline repressor (TetR) from H1 promoter and induce the transcription of siRNA.

The vector also carries a neomycin resistance gene for establishing stable transfection cell line and a cGFP marker for tracking transfection efficiency, both genes are under the control of SV40 promoter. The vector uses CMV enhancer/promoter joined MSV 5’LTR (CMV/MSV 5'LTR) and MSV 3'LTR for viral transcription and packaging.

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