pGBKT7-Lam 载体

基本信息

质粒类型: 酵母双杂交系统
克隆方法: 多克隆位点，限制性内切酶
载体大小: 7.9kb
5' 测序引物及序列: T7 Forward
载体标签: c-Myc
载体抗性: Kanamycin (卡纳霉素)

订购信息

产品编号 产品名称 规格 价格
VT1643 pGBKT7-Lam 2ug 点击询价

质粒图谱

载体描述

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.

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