pJQ200SK 载体

质粒类型: 基因置换载体(自杀型)
克隆方法: 多克隆位点,限制性内切酶
载体抗性: Gentamicin (庆大霉素)
产品编号 产品名称 规格 价格
VT1699 pJQ200SK 2ug 点击询价

Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C Hosts: E.coli.