pIEX/Bac-1 载体

质粒类型: 昆虫细胞表达系统
启动子: IE1, p10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6796 bp (查看载体序列)
载体标签: C-His,N-Strep
载体抗性: Ampicillin (氨苄青霉素)
备注: 拥有hr5增强子
产品编号 产品名称 规格 价格
VT1717 pIEX/Bac-1 2ug 点击询价

The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.

The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.