pLVX-EF1α-mCherry-N1 载体

质粒类型: 慢病毒载体
高拷贝/低拷贝: 高拷贝
启动子: EF1α/ EF1a
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9510 bp (查看载体序列)
3' 测序引物及序列: mCherry-R: TTGGTCACCTTCAGCTTGG
载体标签: c-mCherry
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Puromycin (嘌呤霉素)
备注: 载体能够表达mCherry荧光蛋白
产品编号 产品名称 规格 价格
VT2012 pLVX-EF1α-mCherry-N1 2ug 点击询价

pLVX-EF1α-mCherry-N1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-ofinterest to be fused to the N-terminus of the red fluorescent protein mCherry. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing mCherry fusions, without time-consuming drug and clonal selection.