pLVX-DsRed-Express2-C1 载体

质粒类型: 慢病毒载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8745 bp (查看载体序列)
载体标签: N-DsRed
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: 嘌呤霉素(Puromycin)
备注: 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体,N端含有DsRED荧光蛋白标签。
产品编号 产品名称 规格 价格
VT2020 pLVX-DsRed-Express2-C1 2ug 点击询价

pLVX-DsRed-Express2-C1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to DsRed-Express2, a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed (1). Genes cloned into the multiple cloning site (MCS), located at the C-terminal end of the DsRed-Express2 coding sequence, are expressed as C-terminal DsRed-Express2 fusion proteins. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the DsRed-Express2 coding sequence. Lentiviral particles derived from the vector allow the expression of DsRed-Express2 fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses DsRed-Express2, and may be used to produce marker virus to optimize infection protocols.

pLVX-DsRed-Express2-C1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (2), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-DsRed-Express2-C1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).

In addition to lentiviral elements, pLVX-DsRed-Express2-C1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.


To construct a fusion protein, the gene of interest must be cloned into pLVX-DsRed-Express2-C1 so that it is in-frame with the DsRed-Express2 coding sequence. The inserted sequence does not require an initiation codon (ATG) or a stop codon (TAA, TAG, TGA); however, if you don't want to use the stop codons downstream of the MCS (see map), you can add a stop codon to the end of your gene of interest.

The fusion protein is constitutively expressed when pLVX-DsRed-Express2-C1 is transduced into target cells. Before the vector can be transduced, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (5).