pcDNA3.1(-) 载体

质粒类型: 哺乳动物表达载体
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5427 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin/G418
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
产品编号 产品名称 规格 价格
VT1010 pcDNA3.1(-) 2ug 点击询价

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: • Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells • Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning • Neomycin resistance gene for selection of stable cell lines • Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.