pcDNA3.1/Zeo(-) 载体

基本信息

质粒类型: 哺乳细胞表达载体
启动子: CMV
表达水平: 高
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5014 bp (查看载体序列)
5' 测序引物:   pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:   pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Zeocin (博来霉素)
备注:
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

订购信息

产品编号 产品名称 规格 价格
VT1977 pcDNA3.1/Zeo(-) 2ug 点击询价

质粒图谱

pcDNA3.1/Zeo(-) 质粒图谱

载体描述

This pcDNA™3.1/Zeo(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a reverse-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family

Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

  • Cytomegalovirus (CMV) enhancer-promoter for high-level expression
  • Large multiple cloning site in either forward (+) or reverse (-) orientations
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

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