pGene/v5-His B 载体

质粒类型: 哺乳动物表达载体
启动子: Gal4-E1b
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4654bp (查看载体序列)
5' 测序引物及序列: pGene Fwd: 5'd[CTGCTATTCTGCTCAACCT]3'
载体标签: 6X His, V5
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Zeocin (博来霉素)
产品编号 产品名称 规格 价格
VT1042 pGene/v5-His B 2ug 点击询价

The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:

  • Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
  • Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone