pEF1/myc-His A 载体

别名: pEF1/myc-HisA
质粒类型: 哺乳动物表达载体
表达水平: 高拷贝
启动子: EF-1a
载体大小: 6165 bp (查看载体序列)
5' 测序引物及序列: T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
载体标签: 6X His, myc
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin
产品编号 产品名称 规格 价格
VT1036 pEF1/myc-His A 2ug 点击询价

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.