pET-27b(+) 载体

别名: pET27b, pet 27b
质粒类型: 大肠杆菌表达载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5414 bp (查看载体序列)
5' 测序引物: T7
载体标签: N-pelB, C-His, C-HSV
载体抗性: Kanamycin (卡那霉素)
PelB sequence for periplasmic localization; same as pET25b but kanR
产品编号 产品名称 规格 价格
VT1206 pET-27b(+) 2ug 点击询价

The pET-27b(+) vector (Cat. No. 69863-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance).Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).