pAAV-LacZ 载体

The 7.3 kb pAAV-LacZ is the replication-deficient AAV vector. This vector serves two purposes; first, as a cloning vector; second, as a control vector. As a cloning vector, this vector is cut with NotI, the LacZ cassette is lost and the expression cassette, harboring the gene of interest, from the pCMV-MCS vector will be cloned into the complementing NotI sites of this vector. The recombinant vector will be cotransfected into a viral packaging cell line for production of recombinant, replication-deficient AAV virions. This vector is also a control using the LacZ reporter enzyme.