pCMV-MCS 载体
| 质粒类型: | 腺相关病毒系统 |
|---|---|
| 表达水平: | 高 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4495 bp (查看载体序列) |
| 5' 测序引物及序列: | Beta-globin-F：ATTCTGAGTCCAAGCTAGGC |
| 3' 测序引物及序列: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
| 载体抗性: | Ampicillin (氨苄青霉素) |
The pCMV-MCS is a 4.5 kb cloning vector. The gene of interest is cloned into the multiple cloning site. After cloning, the expression cassette is removed from this vector using the flanking NotI sites for subsequent cloning into the pAAV-LacZ vector.
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