pBABE-Puro 载体

质粒类型: 逆转录病毒载体
载体大小: 5169bp (查看载体序列)
5' 测序引物及序列: pBABE 5': CTTTATCCAGCCCTCAC
3' 测序引物及序列: pBABE 3': ACCCTAACTGACACACATTCC
载体抗性: Ampicillin
筛选标记: Puromycin
产品编号 产品名称 规格 价格
VT1545 pBABE-Puro 2ug质粒 点击询价

Retroviruses are efficient tools for delivering herita ble genes into the genome of dividing cells. Cell Biolabs’ retrovirus vector is based on the pBABE vect or system, which is derived from Moloney murine leukemia virus (MMLV). The vector provides the viral package signal, transcription and processing elements, and a target gene. The viral env gene, produced by the package cell line, encodes the envelop protein, which determines the viral infectivity range . Transfection into a package cell line produces high- titer, replication-incompetent viruses. In addition to transfer and expression of exogenous genes in mammalian cells, recently, retroviruses are used to express silencing RNAs (siRNA) to decrease the expression of target genes both in vitro and in vivo .