pMT/BiP/V5-His A 载体

质粒类型: 昆虫细胞表达载体
启动子: MT
载体大小: 3642 bp (查看载体序列)
载体标签: V5 Epitope Tag, His Tag (6x)
载体抗性: Ampicillin (氨苄青霉素)
产品编号 产品名称 规格 价格
VT1177 pMT/BiP/V5-His A 2ug 点击询价

The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

  • Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
  • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.