pIZT/V5-His 载体

基本信息

质粒类型: 昆虫细胞表达载体
高拷贝/低拷贝: 高拷贝
启动子: OpIE2
克隆方法: 多克隆位点，限制性内切酶
载体大小: 3336bp (查看载体序列)
5' 测序引物及序列: OpIE2：5'd[CGCAACGATCTGGTAAACAC]3'
载体标签: V5 (Cterm)， His (Cterm)
载体抗性: Zeocin (博莱霉素)
筛选标记: Zeocin
备注: Stable expression of gene in insect cells. GFP reporter allows visualization of stable cells.

订购信息

产品编号 产品名称 规格 价格
VT1178 pIZT/V5-His 2ug 点击询价

质粒图谱

pIZTV5-His 质粒图谱

载体描述

载体描述

The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:

  • The OpIE2 promoter for constitutive expression
  • The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
  • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.

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