pRNAT-U6.1/neo 载体
| 质粒类型: | RNAi载体 |
|---|---|
| 启动子: | U6 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6380 bp (查看载体序列) |
| 5' 测序引物及序列: | pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG) |
| 3' 测序引物及序列: | pRNA Reverse (TAGAAGGCACAGTCGAGG) |
| 载体标签: | cGFP |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Neomycin (新霉素) |
pRNAT-U6.1/Neo is designed for mammalian transfection. It carries a Neomycin resistance gene as the selectable marker, which can be used for establishing stable cell line. The GFP marker (coral GFP, cGFP) under CMV promoter control can be used to track the transfection efficiency. It uses U6 promoter for siRNA expression.
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