pGL4.18[luc2P/Neo] 载体

质粒类型: 启动子报告载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5727 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin (新霉素)
备注: Promoterless vector encoding highly destabilized luciferase for measuring the activity of promoter and enhancer sequences.
产品编号 产品名称 规格 价格
VT1732 pGL4.18[luc2P/Neo] 2ug 点击询价

The pGL4.18[luc2P/Neo] Vector(a–e)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.18[luc2P/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with a greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.