pShuttle-IRES-hrGFP-2 载体

质粒类型: 腺病毒系统
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8.9 kb
5' 测序引物及序列: pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
3' 测序引物及序列: pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG
载体抗性: Kanamycin (卡那霉素)
备注: 载体内包含有IRES序列和hrGFP序列
产品编号 产品名称 规格 价格
VT1496 pShuttle-IRES-hrGFP-2 2ug 点击询价

Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).