pGL4.28[luc2CP/minP/Hygro] 载体

质粒类型: 启动子报告载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6061 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Hygromycin (潮霉素)
备注: Promoterless vector encoding highly destabilized luciferase for measuring the activity of promoter and enhancer sequences.
产品编号 产品名称 规格 价格
VT1736 pGL4.28[luc2CP/minP/Hygro] 2ug 点击询价

The pGL4.28[luc2CP/minP/Hygro] Vector(a–f)encodes the luciferase reporter gene luc2CP and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2CP gene. luc2CP is a synthetically derived luciferase sequence with humanized codon optimization. The luc2CP gene also contains hCL1 and hPEST, both of which are protein destabilization sequences. The protein encoded by luc2CP responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coliand a mammalian selectable marker for hygromycin resistance.