| 别名: | pcDNA3.1/myc-His(-) B | 
|---|---|
| 质粒类型: | 哺乳动物表达载体 | 
| 表达水平: | 高拷贝 | 
| 启动子: | CMV | 
| 克隆方法: | 多克隆位点,限制性内切酶 | 
| 载体大小: | 5497 bp (查看载体序列) | 
| 5' 测序引物及序列: | T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' | 
| 载体标签: | 6X His, myc | 
| 载体抗性: | Ampicillin (氨苄青霉素) | 
| 筛选标记: | 新霉素(Neo) | 
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.