pcDNA4/TO/myc-His B 载体

质粒类型: 四环素调控系统
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5155bp (查看载体序列)
载体标签: Myc; 6xHis
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Zeocin
备注: Mammalian cell vector for tetracycline-inducible expression of C-terminally Myc- and 6xHis-tagged proteins.
产品编号 产品名称 规格 价格
VT1596 pcDNA4/TO/myc-His B 2ug 点击询价

A Tetracycline-Regulated Expression System without Viral Transactivators
The T-REx™ System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).

Specific Activation
The T-REx™ System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx™ System elements do not use viral transactivators, you can achieve high-level expression from the complete CMV promoter without secondary, non-specific activation of host genes.

The T-REx™ Mechanism
The T-REx™ transcriptional control elements are illustrated in Figure 1. Two tetracycline operator sequences (TetO2) have been inserted between the TATA box of the CMV promoter and the